Troglitazone Induces Extracellular Matrix and Cytoskeleton Remodeling in Mouse Collecting Duct Cells
Figure 4
Troglitazone attenuates E-cadherin expression in IMCD-K2 and M1 cells. In (a) E-cadherin is detected by immunofluorescence in M1 cells stimulated with DMSO (vehicle, upper) and 10 μM troglitazone (lower) for 24 hours (magnification 100x). In (b) IMCD-K2 and M1 cells were treated with DMSO (vehicle) or troglitazone (TRO), and a representative Western blot is shown for E-cadherin (upper bands) and corresponding β-actin (lower bands). In (c) the corresponding densitometric analysis is shown with data presented as mean ± S.E.M. (*). In (d) a densitometric analysis of E-cadherin is shown for M1 cells treated with 10 μM TRO in the presence or absence of the PPARγ antagonists GW9662 and T0070907. In (e) the densitometric analysis is shown for E-cadherin in M1 cells treated with DMSO or 10 μM TRO with endogenous PPARγ as well as those expressing the control pcDNA plasmid and pcDNA-PPARγ overexpression. Data presented as mean ± S.E.M. (*).
