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Journal of Biomedicine and Biotechnology
Volume 2012, Article ID 510418, 12 pages
http://dx.doi.org/10.1155/2012/510418
Research Article

Quantitative Proteomic Study of Human Lung Squamous Carcinoma and Normal Bronchial Epithelial Acquired by Laser Capture Microdissection

1Key Laboratory of Cancer Proteomics of Chinese Ministry of Health, Xiangya Hospital, Central South University, Changsha 410008, China
2Judicial Police General Hospital, Changsha 410004, China
3Department of Gynecology and Obstetrics, Xiangya Hospital, Central South University, Changsha 410008, China
4Department of Cardiothoracic Surgery, Third Xiangya Hospital, Central South University, Changsha 410013, China
5Department of General Introduction to Surgery, School of Medicine, University of South China, Hengyang 421001, China
6Department of Biology, School of Pharmacy and Life Science, University of South China, Hengyang 421001, China

Received 8 June 2011; Revised 30 November 2011; Accepted 1 December 2011

Academic Editor: Kapil Mehta

Copyright © 2012 Xu Yan et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Objective. To investigate the differential protein profile of human lung squamous carcinoma (HLSC) and normal bronchial epithelium (NBE) and provide preliminary results for further study to explore the carcinogenic mechanism of HLSC. Methods. Laser capture microdissection (LCM) was used to purify the target cells from 10 pairs of HLSC tissues and their matched NHBE, respectively. A stable-isotope labeled strategy using iTRAQ, followed by 2D-LC/Q-STAR mass spectrometry, was performed to separate and identify the differential expression proteins. Results. A total of 96 differential expression proteins in the LCM-purified HLSC and NBE were identified. Compared with NBE, 49 proteins were upregulated and 47 proteins were downregulated in HLSC. Furthermore, the expression levels of the differential proteins including HSPB1, CKB, SCCA1, S100A8, as well as S100A9 were confirmed by western blot and tissue microarray and were consistent with the results of quantitative proteomics. Conclusion. The different expression proteins in HLSC will provide scientific foundation for further study to explore the carcinogenic mechanism of HLSC.