Quantitative Proteomic Study of Human Lung Squamous Carcinoma and Normal Bronchial Epithelial Acquired by Laser Capture Microdissection
Table 2
Differential expressed proteins in LCM-purified HLSC and NHBE identified by mass spectrometry.
No. of proteins
Coverage (%)
UniprotKB
AC
Name
Peptides
115 : 113
1
62.34
IPI00554648.3
P05787
KRT8
60
0.4098
2
60.05
IPI00479145.2
P08727
KRT19
51
0.6193
3
56.53
IPI00745872.2
P02768
ALB
40
0.5735
4
59.8
IPI00009867.3
P13647
KRT5
68
0.7839
5
54.17
IPI00019502.3
P35579
MYH9
32
1.6587
6
39.41
IPI00007752.1
P68371
TUBB2C
37
0.1989
7
35.87
IPI00784154.1
P10809
HSPD1
30
1.5497
8
34.28
IPI00479186.5
P14618
PKM2
21
1.5827
9
34.2
IPI00844215.1
Q13813
SPTAN1
17
0.5947
10
42.7
IPI00450768.7
Q04695
KRT17
25
0.7824
11
30.05
IPI00219018.7
P04406
GAPDH
22
1.5761
12
28.96
IPI00845339.1
P08107
HSPA1A/1B
15
0.5216
13
27.82
IPI00022204.2
P29508
SERPINB3
16
0.3215
14
31.05
IPI00554788.5
P05783
KRT18
32
0.5866
15
50.34
IPI00300725.7
P02538
KRT6A
59
1.6359
16
24.25
IPI00553177.1
P01009
SERPINA1
13
0.651
17
23.08
IPI00654755.3
P68871
HBB
19
1.4246
18
22.65
IPI00455383.4
Q00610
CLTC
12
0.6788
19
23.9
IPI00003362.2
P11021
HSPA5
13
1.9385
20
20.99
IPI00025252.1
P30101
PDIA3
14
1.477
21
19.32
IPI00218914.5
P00352
ALDH1A1
11
0.3403
22
35.01
IPI00217963.3
P08779
KRT16
19
1.7771
23
18.49
IPI00872684.1
P15311
EZR
9
0.3327
24
16.7
IPI00013808.1
O43707
4ACTN4
14
0.4979
25
33.95
IPI00290077.1
P19012
KRT15
20
0.6399
26
16
IPI00219757.13
P09211
GSTP1
13
0.5584
27
15.06
IPI00000105.4
Q14764
MVP
7
0.516
28
14.96
IPI00645078.1
P22314
UBA1
8
1.345
29
14.66
IPI00788802.1
P29401
TKT
8
1.4856
30
13.96
IPI00020599.1
P27797
CALR
7
2.1548
31
13.57
IPI00169383.3
P00558
PGK1
8
0.6459
32
13.23
IPI00887678.1
LOC654188
8
2.2579
33
12.44
IPI00640741.1
Q06830
PRDX1
6
6.5599
34
12.12
IPI00604620.3
P19338
NCL
7
1.5576
35
12.1
IPI00893881.1
Q53G64
AGR2
14
0.4388
36
11.27
IPI00853068.1
Q86YQ1
HBA
11
1.372
37
11.23
IPI00816252.1
H2BFS
18
1.4498
38
11.14
IPI00657682.2
P08263
GSTA1
6
0.2536
39
11.01
IPI00643384.2
A6NLG9
BGN
5
0.2803
40
23.25
IPI00550661.2
P13646
KRT13
20
2.3013
41
38.63
IPI00384444.5
P02533
KRT14
19
1.448
42
10.4
IPI00027497.5
P06744
GPI
4
2.4216
43
9.95
IPI00027462.1
P06702
S100A9
7
3.1824
44
9.85
IPI00215948.4
P35221
CTNNA1
5
0.6596
45
9.76
IPI00453473.6
P62805
HIST1H4I
5
15.7322
46
9.42
IPI00794543.1
A8K1M2-cDNA
FLJ75174
4
0.3772
47
9.08
IPI00549248.4
P06748
NPM1
9
2.033
48
8.97
IPI00221226.7
P08133
ANXA6
4
0.6198
49
8.63
IPI00032140.4
P50454
SERPINH1
4
2.9965
50
8.55
IPI00025512.2
P04792
HSPB1
20
3.9981
51
8.14
IPI00798430.1
Q53H26
TF
4
0.6764
52
35.45
IPI00011654.2
P07437
TUBB
28
1.5243
53
8
IPI00022977.1
P12277
CKB
4
0.41
54
7.84
IPI00307162.2
P18206
VCL
5
2.4223
55
7.22
IPI00295400.1
P23381
WARS
3
1.4314
56
6.92
IPI00792011.1
Q8NF12
CAPS
5
0.3241
57
6.36
IPI00013415.1
P62081
RPS7
3
3.2407
58
6.27
IPI00025465.1
P20774
OGN
4
0.1986
59
6.24
IPI00853455.1
C9JIH9
CTSD
4
0.639
60
6.19
IPI00152295.1
Q8TC71
SPATA18
3
0.4421
61
9.34
IPI00829767.1
P01859
IGHG2
4
0.6251
62
6
IPI00789551.1
A8MXP9
MATR3
5
2.0326
63
6
IPI00871851.1
Q15019
SEPT2
4
1.3502
64
6
IPI00291006.1
P40926
MDH2
3
1.924
65
6
IPI00795633.1
Q8IWL5
CLU
3
0.3392
66
5.95
IPI00423462.5
Q6N092
IGHA1
3
0.7002
67
8.33
IPI00007047.1
P05109
S100A8
5
2.1281
68
5.73
IPI00654709.1
Q6PKA6
ALDH3A1
3
0.431
69
4.72
IPI00294834.6
Q12797
ASPH
2
2.3432
70
6.6
IPI00027350.3
P32119
PRDX2
3
1.7333
71
4.45
IPI00025086.3
P20674
COX5A
2
1.4661
72
4.4
IPI00009032.1
P05455
SSB
2
0.6693
73
4.4
IPI00060715.1
Q96CX2
KCTD12
2
0.7126
74
4.31
IPI00646059.1
Q5VXV2
SET
2
2.3791
75
4.13
IPI00216308.5
P21796
VDAC1
2
0.4488
76
4.08
IPI00032179.2
P01008
SERPINC1
2
0.6734
77
4.01
IPI00025796.3
O75489
NDUFS3
2
0.6022
78
4
IPI00020042.2
P43686
PSMC4
2
0.603
79
4
IPI00011126.6
P62191
PSMC1
2
0.6418
80
3.71
IPI00883950.1
Q8TB01
CKAP4
2
2.3486
81
2.95
IPI00420014.2
O75643
ASCC3L1
1
1.6656
82
2.73
IPI00003944.1
P11182
DBT
1
0.5156
83
2.58
IPI00479786.4
Q92945
KHSRP
1
1.9958
84
2.38
IPI00419237.3
P28838
LAP3
1
1.7329
85
2.34
IPI00024933.3
P30050
RPL12
1
1.7853
86
2.19
IPI00219221.3
P47929
LGALS7
1
0.7374
87
2.02
IPI00444204.1
Q6ZS74
ALB
1
2.0254
88
2.02
IPI00847322.1
Q4ZJI1
SOD2
1
0.6501
89
2.01
IPI00745729.2
A6PVW9
SELENBP1
1
0.5016
90
2.01
IPI00375746.4
Q6ZN66
GBP6
1
4.68
91
2
IPI00413654.3
Q13243
SFRS5
1
4.0553
92
2
IPI00010133.3
P31146
CORO1A
1
1.5283
93
2
IPI00221222.7
P53999
SUB1
1
7.5809
94
2
IPI00024976.5
Q9NS69
TOMM22
1
2.5511
95
2
IPI00888660.1
SDHA
1
2.0706
96
2
IPI00013895.1
P31949
S100A11
2
0.6028
Differentially labeled proteins are identified by QSTAR. The % coverage of analyzed peptides and Swiss-Prot accession number were shown for each protein. Proteins displaying an average fold-difference of ≥1.2-fold up- (+) or ≤0.8-fold down- (-) regulation between pairs of conditions where are shown (115: the group of HLSC; 113: the group of NHBE).
