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Journal of Biomedicine and Biotechnology
Volume 2012 (2012), Article ID 604076, 9 pages
Research Article

Distribution of Genes and Repetitive Elements in the Diabrotica virgifera virgifera Genome Estimated Using BAC Sequencing

1Corn Insects and Crop Genetics Research Unit, ARS, USDA, Ames, IA 50011, USA
2Department of Entomology, Iowa State University, Ames, IA 50011, USA
3Department of Entomology, University of Nebraska, Lincoln, NE 68583, USA
4University of Illinois, Champaign-Urbana, IL 61801, USA
5North Central Agricultural Research Laboratory, Brookings, ARS, USDA, SD 57006, USA

Received 29 February 2012; Accepted 16 May 2012

Academic Editor: Yong Lim

Copyright © 2012 Brad S. Coates et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Supplementary Material

Figure S1: Annotation of end sequences showing assigned gene ontologies by assignments for biological process (P), cellular complement (C), and molecular function (F) are shown at GO level 2 (Note: a given sequence read may be assigned > 1 GO term).

Figure S2: Annotations assigned BAC end sequence data. Results from queries of the NCBI non-redundant (nr) database conducted using the blastx (panel A) and blastn algorithms are reported (panel B). Top blast hits are shown for those with E-values ≥ 10-20. The “Diabrotica cadherin intron region” category consists of predicted introns with the D. v. virgifera cadherin gene sequence (EF531715.1). “Diabrotica microsatellite” category refers to identified microsatellite loci Dviz14 (EF524289.1), Dvaz 12 (EF524287.1), Dba07 (EF524283.1), Dba08 (EF524282.1), DVV-D12 (AY738542.1), DVV-ED11 (DQ867092.1), DVV-ED14 (DQ867094.1), and DVV-ET10 (DQ867100.1).

Table S1: List of functional annotations for Diabrotica virgifera virgifera BAC end sequences.

  1. Supplementary Figures
  2. Supplementary Table