Improvement of Coenzyme Q10 Production: Mutagenesis Induced by High Hydrostatic Pressure Treatment and Optimization of Fermentation Conditions

<table>Genealogy of mutant strains derived from <i>AT </i>1.2554. Mutation markers: <svg height="15.475" id="M12" style="vertical-align:-3.25793pt" version="1.1" viewbox="0 0 39.712502 15.475" width="39.712502" xmlns="http://www.w3.org/2000/svg">
<g transform="matrix(1.25,0,0,-1.25,0,15.475)">
<g transform="translate(72,-59.62)">
<text transform="matrix(1,0,0,-1,-71.95,62.92)">
<tspan style="font-size: 12.50px; " x="0" y="0">N</tspan>
<tspan style="font-size: 12.50px; " x="8.8021116" y="0">a</tspan>
<tspan style="font-size: 12.50px; " x="14.353444" y="0">N</tspan>
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<text transform="matrix(1,0,0,-1,-48.57,59.8)">
<tspan style="font-size: 8.75px; " x="0" y="0">3</tspan>
</text>
<text transform="matrix(1,0,0,-1,-43.7,67.92)">
<tspan style="font-size: 8.75px; " x="0" y="0">r</tspan>
</text>
</g>
</g>
</svg>, sodium azide (NaN<sub>3</sub>) resistance; Tyr<sup>-</sup>, tyrosine auxotroph; <svg height="11.8875" id="M13" style="vertical-align:-0.12538pt" version="1.1" viewbox="0 0 26.1 11.8875" width="26.1" xmlns="http://www.w3.org/2000/svg">
<g transform="matrix(1.25,0,0,-1.25,0,11.8875)">
<g transform="translate(72,-62.49)">
<text transform="matrix(1,0,0,-1,-71.95,62.67)">
<tspan style="font-size: 12.50px; " x="0" y="0">E</tspan>
<tspan style="font-size: 12.50px; " x="7.6393328" y="0">t</tspan>
<tspan style="font-size: 12.50px; " x="11.115167" y="0">h</tspan>
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<text transform="matrix(1,0,0,-1,-54.58,67.92)">
<tspan style="font-size: 8.75px; " x="0" y="0">r</tspan>
</text>
</g>
</g>
</svg>, ethionine resistance; <svg height="11.5625" id="M14" style="vertical-align:-0.12538pt" version="1.1" viewbox="0 0 30.424999 11.5625" width="30.424999" xmlns="http://www.w3.org/2000/svg">
<g transform="matrix(1.25,0,0,-1.25,0,11.5625)">
<g transform="translate(72,-62.75)">
<text transform="matrix(1,0,0,-1,-71.95,62.92)">
<tspan style="font-size: 12.50px; " x="0" y="0">D</tspan>
<tspan style="font-size: 12.50px; " x="9.0271664" y="0">a</tspan>
<tspan style="font-size: 12.50px; " x="14.578498" y="0">u</tspan>
</text>
<text transform="matrix(1,0,0,-1,-51.12,67.92)">
<tspan style="font-size: 8.75px; " x="0" y="0">r</tspan>
</text>
</g>
</g>
</svg> daunomycin resistance; <svg height="15.475" id="M15" style="vertical-align:-3.25793pt" version="1.1" viewbox="0 0 33.049999 15.475" width="33.049999" xmlns="http://www.w3.org/2000/svg">
<g transform="matrix(1.25,0,0,-1.25,0,15.475)">
<g transform="translate(72,-59.62)">
<text transform="matrix(1,0,0,-1,-71.95,62.92)">
<tspan style="font-size: 12.50px; " x="0" y="0">V</tspan>
<tspan style="font-size: 12.50px; " x="9.0271664" y="0">K</tspan>
</text>
<text transform="matrix(1,0,0,-1,-53.9,59.8)">
<tspan style="font-size: 8.75px; " x="0" y="0">3</tspan>
</text>
<text transform="matrix(1,0,0,-1,-49.02,67.92)">
<tspan style="font-size: 8.75px; " x="0" y="0">r</tspan>
</text>
</g>
</g>
</svg>, vitamin K<sub>3</sub> resistance. All the mutants were induced by HHP or UV + DES treatment. The specific CoQ10 content (mg/g dry cell weight) was obtained in flask tests.</table>

BioMed Research International

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Figure 2

Figure 2: Improvement of Coenzyme Q10 Production: Mutagenesis Induced by High Hydrostatic Pressure Treatment and Optimization of Fermentation Conditions 

Figure 2 | Improvement of Coenzyme Q10 Production: Mutagenesis Induced by High Hydrostatic Pressure Treatment and Optimization of Fermentation Conditions 