Differential Control of Growth, Apoptotic Activity, and Gene Expression in Human Breast Cancer Cells by Extracts Derived from Medicinal Herbs Zingiber officinale
Ginger extract modulates expression of the apoptosis-related proteins. The MCF-7 and MDA-MB-231 cells were incubated with the indicated concentrations of the ethanol extract of ginger for 24 h. (a) Cell lysates were prepared and examined for the expression of Bcl-2 and Bax proteins by western blot as described in the Materials and Methods. -actin was used as the internal control. Representative blots from three independent experiments are shown. (b) The total RNAs were isolated, reverse transcribed, and subjected to PCR with the Bcl-2- and Bax-specific primers. The PCR products were then subjected to electrophoresis in 1% agarose gel and visualized by EtBr staining. HPRT was used as the internal control. A typical result from three independent experiments is shown. (c) The histogram depicts the Bcl-2 (dark bars) and Bax (light bars) mRNA ratio in MCF-7 and MDA-MB-231 cells measured by using densitometric analysis, (*); (#).