High-Resolution Whole-Mount In Situ Hybridization Using Quantum Dot Nanocrystals
Figure 2
QD-streptavidin staining of LTBP1 compares favorably with the published staining achieved using standard chromogenic protocols in the deeper structures of the embryo [1]. (a) A transverse section from a whole mount in situ indicating the LTBP1 transcript expression pattern using QD705 nm streptavidin. The somitic staining obtained using QDs is identical to the published data using the chromogenic protocol [1], showing that the QD-streptavidin solution can penetrate and stain the deep areas of the somites. (b) A series of transverse optical sections of a QD-streptavidin-stained embryo for the LTBP1 message. The optical sections reveal that the staining previously identified as notochord by the chromogenic protocol is in fact somitic mesoderm flanking the notochord (nc: notochord, sm: somatic mesoderm).
