Evaluation of Distinct Freezing Methods and Cryoprotectants for Human Amniotic Fluid Stem Cells Cryopreservation

Table 1

RT-PCR primer sequences.

Gene	Primers	PCR products (bp)	Tm (°C)
Oct-4	5′- cgt gaa gct gga gaa gga gaa gct g -3′; 5′- caa ggg ccg cag ctt aca cat gtt c-3′	247	61
Nanog	5′-cat gag tgt gga tcc agc ttg-3′; 5′-cct gaa taa gca gat cca tgg-3′	191	52
Osteocalcin	5′-atg aga gcc ctc aca ctc ct-3′; 5′ caa ggg gaa gag gaa aga ag-3′	359	55
Osteopontin	5′-cat ggc atc acc tgt gcc ata cc-3′; 5′-cag tga cca gtt cat cag att cat c-3′	331	57
Wnt-1	5′-cac gac act cgt gca gta cgc-3′; 5′-aca gac act cgt gca gta cgc-3′	233	58
Collagen 2A1	5′-cca gga cca aag gga cag aaa g-3′; 5′-ttc acc agg ttc acc agg att g-3′	398	57
Perlecan	5′-cat aga gac cgt cac agc aag-3′; 5′-atg aac acc aca ctg aca acc-3′	300	55
Syndecan	5′-cct tca cac tcc cca cac-3′; 5′-ggc ata gaa ttc ctc ctg ttg-3′	410	54
PPAR-γ 2	5′-gct gtt atg ggt gaa act ctg-3′; 5′-ata agg tgg aga tgc agg ctc-3′	351	55
Lipoprotein lipase	5′-cag caa aac ctt ca tggt-3′; 5′-agt ttt ggc acc caa ctc tca-3′	72	53
β-actin	5′-tgg cac cac acc ttc tac aat gag c-3′; 5′-gca cag ctt ctc ctt aat gtc acg c-3′	396	60