Structure and Functional Characteristics of Rat’s Left Ventricle Cardiomyocytes under Antiorthostatic Suspension of Various Duration and Subsequent Reloading
Table 2
Transversal stiffness (pN/nm) of isolated rat’s permeabilized left ventricular myocytes in liquid in relaxed, calcium activated (pCa = 4.2) and rigor states under gravitational unloading and subsequent reloading.
State
Group
Relaxed
Activation (pCa = 4.2)
Rigor
Transversal stiffness of sarcolemma between the M-band and Z-disk projections
Control ()
4.03 ± 0.11
9.1 ± 0.4@
9.67 ± 0.24@
1-HS ()
4.80 ± 0.22*
9.5 ± 0.5@
10.1 ± 0.3@
3-HS ()
6.22 ± 0.29*
10.2 ± 0.3*/@
10.8 ± 0.4*/@
7-HS ()
7.79 ± 0.12*
10.5 ± 0.3*/@
13.3 ± 0.4*/@
14-HS ()
12.3 ± 0.4*
12.9 ± 0.4*
13.3 ± 0.3*/@
14-HS + 3-R ()
6.4 ± 0.5*/$
7.5 ± 0.3*/$
8.4 ± 0.6*/$/@
14-HS + 7-R ()
4.3 ± 0.4
8.9 ± 0.5@
9.2 ± 0.3@
Transversal stiffness of sarcolemma at the M-band projection
Control
2.85 ± 0.12
6.8 ± 0.5@
7.4 ± 0.6@
1-HS
2.68 ± 0.17
6.6 ± 0.3@
7.0 ± 0.3@
3-HS
3.77 ± 0.25*
6.7 ± 0.4@
7.7 ± 0.5@
7-HS
5.79 ± 0.19*
6.9 ± 0.3@
7.9 ± 0.3@
14-HS
7.55 ± 0.13*
7.9 ± 0.6*
9.4 ± 0.3*/@
14-HS + 3-R
3.57 ± 0.20*/$
5.9 ± 0.4$/@
6.3 ± 0.3$/@
14-HS + 7-R
3.1 ± 0.3
6.2 ± 0.4@
7.0 ± 0.6@
Transversal stiffness of sarcolemma at the Z-disk projection
Control
10.0 ± 0.3
14.5 ± 1.2@
15.3 ± 0.5@
1-HS
10.7 ± 0.8
15.4 ± 0.3@
16.3 ± 0.5@
3-HS
10.6 ± 0.3
14.8 ± 0.3@
15.2 ± 0.7@
7-HS
10.4 ± 0.3
11.9 ± 0.3@
16.5 ± 0.3@
14-HS
17.2 ± 0.4*
17.9 ± 0.4*
18.2 ± 0.5*/@
14-HS + 3-R
9.2 ± 0.3*/$
10.3 ± 0.6*/$
11.9 ± 0.5*/$/@
14-HS + 7-R
9.9 ± 0.7
15.1 ± 0.8@
15.6 ± 0.4@
as compared to the analogous state of the group “Control”, @ as compared to the relaxed state at the same group, $ as compared to the analogous state of the group “14-HS”.
