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Journal of Biomedicine and Biotechnology
Volume 2012, Article ID 715928, 10 pages
http://dx.doi.org/10.1155/2012/715928
Research Article

Quantitation of Pyrrole-Imidazole Polyamide in Rat Plasma by High-Performance Liquid Chromatography Coupled with UV Detection

1Department of Clinical Pharmacokinetics, School of Pharmacy, Nihon University, 7-7-1 Narashinodai, Chiba, Funabashi City 274-8555, Japan
2Department of Clinical Pharmacotherapy, School of Pharmacy, Nihon University, 7-7-1 Narashinodai, Chiba, Funabashi City 274-8555, Japan
3Chiba Cancer Center Research Institute, 666-2, Nitonacho, Chuo-ku, Chiba, Chiba City 260-8717, Japan
4Department of Medicine, School of Medicine, Nihon University, Tokyo 173-8610, Japan

Received 9 March 2012; Accepted 16 April 2012

Academic Editor: John B. Vincent

Copyright © 2012 Tomonori Kamei et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

A simple and robust method using high-performance liquid chromatography with UV detection was developed and validated for the determination of six pyrrole-imidazole (PI) polyamides (HN.49, TGF-β1f, TGF-β1t, HN.50f, HN.50t, and LOX-1) in rat plasma. After the plasma proteins were precipitated with methanol containing phenacetin as an internal standard, the analytes were separated on a Luna C18 (2) (5 μm, 4 . 6 × 1 5 0  mm). Calibration curves were linear over the range of 0.5 to 200 μg/mL for HN.49, 0.25 to 200 μg/mL for TGF-β1f, TGF-β1t, HN.50t, and LOX-1, 1 to 200 μg/mL for HN.50f in rat plasma. The inter- and intraday precision were below 15%, and the accuracy was within 15% at the quality controls. The validated method was successfully applied to sample analysis for the pharmacokinetic study.