The Plasminogen Activation System and the Regulation of Catecholaminergic Function

Figure 10

Overexpression of Plg-R_KT increases cell surface plasminogen activation. PC12 cells stably overexpressing Plg-R_KT (pCIneo-Plg-R_KT) (a) or vector alone (pCIneo) (b) were analyzed by dual color FACS analysis. Viable cells were gated from nonviable cells and histogram plots of viable cells are shown. Dotted tracings: anti-Plg-R_KT mAb IgG. Black tracings: isotype control IgG. Dashed tracings: autofluorescence. (c) PC12 cells stably overexpressing Plg-R_KT (pCIneo-Plg-R_KT) or vector alone (pCIneo) were incubated with plasminogen and t-PA was added and plasminogen activation was measured. Cell-mediated plasminogen activation was substantially greater in Plg-R_KT overexpressing cells than in control cells (𝑃<0.001 at each cell concentration tested) (𝑛=3). [25]. ©The American Society for Biochemistry and Molecular Biology.

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