Figure 11: Effect of overexpression of Plg-RKT on catecholamine release. PC12 cells stably overexpressing either Plg-RKT (filled bars) or vector alone (open bars) were treated with 60 μM nicotine (Nicotine) or buffer (Basal) at 37°C for 15 minutes and catecholamine release was measured by liquid scintillation counting. Percent release was calculated as percentage of secretion [amount released/(amount released + amount in cell lysate)] and results expressed as net release (% secretagogue-stimulated release minus % basal release). Results are represented as mean ± SEM, 𝑛 = 9 for each experimental group. 𝑃 < 0 . 0 0 1 for the Plg-RKT transfectants stimulated with nicotine compared with corresponding values for the vector control cells. [25]. ©The American Society for Biochemistry and Molecular Biology.