Figure 9: Plg-RKT is an integral plasma membrane cell surface protein. (a) Phase partitioning of Plg-RKT. An immunoreactive band corresponding to the Mrapp of Plg-RKT was detected in the detergent phase, but not in the aqueous phase. (b) Coimmunoprecipitation of Plg-RKT with uPAR. (c) FACS analysis of Plg-RKT expression on intact PC12 cells. Viable cells were gated from nonviable cells and histogram plots of viable cells are shown. Dotted tracings: anti-Plg-RKT mAb IgG. Black tracings: isotype control IgG. Dashed tracings: autofluorescence. [25]. ©The American Society for Biochemistry and Molecular Biology.