Figure 3: Per base sequence quality of sample 2 as generated by FASTQC for the dataset obtained from RTA base calling (a) and OLB base calling (b). The yellow box plots (red bar: median, box: interquartile ranges 25–75%, and whisker: 10–90% percentile) show the base-calling quality scores across all sequencing reads of sample 2. The blue line indicates the mean quality score. The other samples had similar per base sequence quality.