Methodology Report

Technical Considerations for Reduced Representation Bisulfite Sequencing with Multiplexed Libraries

Table 2

Details of data generated for multiplexed RRBS libraries.

Sample IDAdaptor
index ID
Raw data including cif files (Gb)1After RTA base calling (Gb)1Sequence volume (Gb)2Uncompressed, 2 reads (Gb)Paired-end reads (106)

113.308.632.8
234.9912.949.4
3832055.36.0615.760.0
492.787.227.5
5104.2110.941.7

1RTA uses the cif files to perform the base calling and produce. bcl files; the samples are not demultiplexed at this stage.
2CASAVA performs the demultiplexing and uses the. bcl files to generate FASTQ files for each of the samples.