Figure 9: Two-dimensional gel analysis of L. interrogans PLG binding proteins. Leptospiral total cell lysates were separated by isoelectric point (pH 3–10) and molecular mass on 12% SDS-PAGE. Replicate gels were Coomassie Blue stained (a) or transferred to membranes and submitted to PLG affinity immunoblotting (b). The reactive spots are enumerated on (a). The results are representative of three independent experiments performed in duplicates.