A Limited Role of p53 on the Ability of a Hexane Fraction of American Ginseng to Suppress Mouse Colitis
Figure 2
The Hexane fraction of AG (HAG) drives apoptosis (TUNEL+) of lymphoblasts marginally better in p53+/+ cells compared with p53−/− cells. The TK6 human lymphoblastoid line, NH32 isogenic p53 knockout cells, and Jurkat T cells (dysfunctional p53 pathway) were cultured in RPMI-1640 + 10% NBCS. Cells were exposed to HAG (in triplicate, per treatment) in RPMI-1640 + 0.1% NBCS for 24 hr at indicated doses and harvested for TUNEL apoptotic assay. The percentage of the cells (Mean ± S.E.) staining positive for TUNEL, indicating apoptosis are given (): (a) TK6 cells; (b) NH32 cells; (c) Jurkat T cells. A minimum of 20,000 events was counted by flow cytometry from each treatment. (d) Confirmation of p53 protein status of the TK6, NH32, and Jurkat T cells. Cell lysates were analyzed by western blot analysis. C+ indicates the positive control for p53, which is an archived HCT-116 cell lysate with wild-type p53. C−, indicates the negative control for p53, which is an archived HCT-116 p53−/− cell lysate with p53 knockout. Results indicate HAG induces apoptosis in lymphoblasts through a limited p53 activity. Significant differences are indicated, where *-value <0.05 and **-value <0.005, when compared to the control (0 μg/mL) treatment.
