Interaction between Pheromone and Its Receptor of the Fission Yeast Schizosaccharomyces pombe Examined by a Force Spectroscopy Study
Figure 2
(a) Typical retraction force curves between P-factor and the cells. Vertical axis indicates cantilever deflections as a function of the cantilever-sample retraction distance. The first curve from the top shows no interaction, and the other curves display specific unbinding events between peptide and the cell. The vertical force jump of events allows access to the specific unbinding force. (b) Force histogram of unbinding events (blue columns) obtained after analysis of 1024 force curves. The mean unbinding force is 120 pN, for a mean loading rate of 1.74 μm/s. In the presence of free 1 μM P-factor, the number of unbinding events decreased (red columns).
