Comparison of Two Suspension Arrays for Simultaneous Detection of Five Biothreat Bacterial in Powder Samples

Figure 4

Specificity test of probes in multiplex primer PCR-coupled suspension array. For each tested agent (𝑥-axis), the response of each of six beads is shown (𝑦-axis). Response is given as the median fluorescent intensity (MFI) at 𝑧-axis. Dotted bars indicate the probe of BA-1-coated bead; grid bars correspond to BA-2; right-slash bars are Yp-2; straight bars are Bru-2; right-dot bars indicate Ft-2; zigzag bars are Bp-2. From the 𝑦-axis, B is the blank blocks; Ba is Bacillus anthracis Sterne; Yp is Yersinia pestis EV76; Bru is Brucella spp. M5; Ft is Francisella tularensis and Bp is Burkholderia pseudomallei. The six bacterial coated beads are from species Bacillus anthracis, Yersinia pestis, Brucella abortus, Francisella tularensis, and Burkholderia pseudomallei, respectively. Each sample yields an appropriate response from each of the six beads present.