Identification of HLA-A24-Restricted Novel T Cell Epitope Peptides Derived from P-Cadherin and Kinesin Family Member 20A
Figure 3
IFN-γ production by CTL clones responding to COS7 cells that expressing HLA-A*2402 and respective oncogene. (a) IFN-γ production by CDH3-10-807 peptide-specific CTL clone when exposed with COS7 cells expressing both HLA-A*2402 and CDH3 (closed diamond), HLA-A*2402 (open triangle), or CDH3 (open circle). (b) IFN-γ production by KIF20A-10-66 peptide-specific CTL clone when exposed with COS7 cells expressing HLA-A*2402 and KIF20A (closed diamond), HLA-A*2402 (open triangle), or KIF20A (open circle). Both CTL clones significantly produced IFN-γ responding to COS7 cells expressing HLA-A*2402 and corresponding gene. Similar results were obtained in three independent experiments. Independently induced other CTL clones also produced significant amount of IFN-γ when exposed with COS7 cell expressing both HLA-A*2402 and respective gene (data not shown). R/S ratio, responder cell (CTL clone)/stimulator cell (COS7 cell) ratio.
