Identification of HLA-A24-Restricted Novel T Cell Epitope Peptides Derived from P-Cadherin and Kinesin Family Member 20A
Figure 6
Cytotoxic activity against tumor cells expressing respective gene. (a) The expression of CDH3 and KIF20A protein in tumor cells used in cytotoxic assay. (b) Cytotoxic activity of CDH3-10-807 peptide-specific CTL clone against CDH3-expressing HLA-A*2402-positive PK-45P cells (closed diamond), CDH3-expressing HLA-A*2402-negative H358 cells (open square), or MiaPaCa-2 cells (open circle). Cytotoxicity against PK-45P cells was significantly higher than those against other cells. (c) Cytotoxic activity of KIF20A-10-66 peptide-specific CTL clone against KIF20A-expressing HLA-A*2402-positive MKN-45 cells (closed diamond), KIF20A-expressing HLA-A*2402-negative PK-59 cells (open square), or MiaPaCa-2 cells (open circle). Cytotoxicity against MKN-45 cells or PK-59 cells are significantly higher than that against MiaPaCa-2 cells, although cytotoxic activity against PK-59 cells was significantly lower compared with that against MKN-45 cells. E/T ratio, effector cell (CTL clone)/target cell (tumor cell) ratio. Similar results were obtained in three independent experiments using same CTL clone and in independent experiments using other CTL clones.
