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Journal of Biomedicine and Biotechnology
Volume 2012, Article ID 902351, 7 pages
http://dx.doi.org/10.1155/2012/902351
Research Article

Treatment with Aqueous Extract from Croton cajucara Benth Reduces Hepatic Oxidative Stress in Streptozotocin-Diabetic Rats

1Post-Graduation Program in Medical Sciences, Medical School, Federal University of Rio Grande do Sul (UFRGS), 90035-903 Porto Alegre, RS, Brazil
2Research Center, Hospital de Clínicas de Porto Alegre, Federal University of Rio Grande do Sul (UFRGS), 90035-903 Porto Alegre, RS, Brazil
3Physiotherapy Course, Catholic University of Pelotas (UCPEL), Porto Alegre, RS, Brazil
4Federal University of Health Sciences of Porto Alegre (UFCSPA), 90050-170 Porto Alegre, RS, Brazil
5Lutheran University of Brazil, 92425-900 Canoas, RS, Brazil
6State University of Rio Grande do Sul Porto Alegre, 90010-191 Porto Alegre, RS, Brazil

Received 12 March 2012; Revised 1 May 2012; Accepted 7 May 2012

Academic Editor: Sandro Massao Hirabara

Copyright © 2012 Graziella Ramos Rodrigues et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Croton cajucara Benth is a plant found in Amazonia, Brazil and the bark and leaf infusion of this plant have been popularly used to treat diabetes and hepatic disorders. The present study was designed to evaluate the oxidative stress as well as the therapeutic effect of Croton cajucara Benth (1.5 mL of the C. cajucara extract i.g.) in rats with streptozotocin-induced diabetes. Croton cajucara Benth was tested as an aqueous extract for its phytochemical composition, and its antioxidant activity in vitro was also evaluated. Lipid peroxidation and superoxide dismutase, catalase, and glutathione reductase activities were measured in the hepatic tissue, as well as the presence activation of p65 (NF-κB), through western blot. Phytochemical screening of Croton cajucara Benth detected the presence of flavonoids, coumarins and alkaloids. The extract exhibited a significant antioxidant activity in the DPPH-scavenging and the hypoxanthine/xanthine oxidase assays. Liver lipid peroxidation increased in diabetic animals followed by a reduction in the Croton-cajucara-Benth-treated group. There was activation of p65 nuclear expression in the diabetic animals, which was attenuated in the animals receiving the Croton cajucara Benth aqueous extract. The liver tissue in diabetic rats showed oxidative alterations related to the streptozotocin treatment. In conclusion the Croton cajucara Benth aqueus extract treatment effectively reduced the oxidative stress and contributed to tissue recovery.