Effects of Ginsenoside 𝐑𝐛_𝟏 on Skin Changes

Table 5

Effects of ginsenoside Rb₁ on the numbers of apoptotic and 8-OHdG-positive cells at week 12 in the skin of UVB-irradiated hairless mice [31].

	Apoptotic cells (number/field)	8-OHdG-positive cells (number/field)
Normal mice	0±0 *	106±7 *
Vehicle-treated UVB-irradiated mice (control)	102±10	286±32
+Ginsenoside Rb1
(100 fg/mouse)	19±11 *	150±24 *
(10 pg/mouse)	11±11 *	183±27 *
(1 ng/mouse)	9±9 *	109±26 *

Ginsenoside Rb₁ (100 fg, 10 pg, and 1 ng/mouse) was applied topically to the dorsal region of each mouse every day for 12 weeks. The expression levels of apoptotic cells and 8-hydroxy-2′-deoxyguanosine (8-OHdG) (marker of oxidative DNA damage) in the dorsal skin of UVB-irradiated hairless mice were examined by the TUNEL method using an apoptosis in situ detection kit and an immunoperoxidase technique using anti-8-OHdG antibody.
Values are the mean ± SE for 6 mice. *Significantly different from UVB-irradiated mice (control), 𝑃<0.05.