Table 5: Effects of ginsenoside Rb1 on the numbers of apoptotic and 8-OHdG-positive cells at week 12 in the skin of UVB-irradiated hairless mice [31].

Apoptotic cells (number/field)8-OHdG-positive cells (number/field)

Normal mice 0 ± 0 * 1 0 6 ± 7 *

Vehicle-treated UVB-irradiated mice (control) 1 0 2 ± 1 0 2 8 6 ± 3 2

+Ginsenoside Rb1
 (100 fg/mouse) 1 9 ± 1 1 * 1 5 0 ± 2 4 *
  (10 pg/mouse) 1 1 ± 1 1 * 1 8 3 ± 2 7 *
  (1 ng/mouse) 9 ± 9 * 1 0 9 ± 2 6 *

Ginsenoside Rb1 (100 fg, 10 pg, and 1 ng/mouse) was applied topically to the dorsal region of each mouse every day for 12 weeks. The expression levels of apoptotic cells and 8-hydroxy-2′-deoxyguanosine (8-OHdG) (marker of oxidative DNA damage) in the dorsal skin of UVB-irradiated hairless mice were examined by the TUNEL method using an apoptosis in situ detection kit and an immunoperoxidase technique using anti-8-OHdG antibody.
Values are the mean ± SE for 6 mice. *Significantly different from UVB-irradiated mice (control), 𝑃 < 0 . 0 5 .