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Journal of Biomedicine and Biotechnology
Volume 2012, Article ID 973140, 8 pages
Research Article

BAC-Dkk3-EGFP Transgenic Mouse: An In Vivo Analytical Tool for Dkk3 Expression

1JST, CREST, 3-2 Yamadaoka, Osaka, Suita 565-0871, Japan
2Department of Developmental Biology, Osaka Bioscience Institute, 6-2-4 Furuedai, Osaka, Suita 565-0874, Japan
3Laboratory for Molecular and Developmental Biology, Institute for Protein Research, Osaka University, Suita 565-0871, Japan

Received 15 February 2012; Revised 19 April 2012; Accepted 3 May 2012

Academic Editor: Thomas Lufkin

Copyright © 2012 Yuki Muranishi and Takahisa Furukawa. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


Dickkopf (DKK) family proteins are secreted modulators of the Wnt signaling pathway and are capable of regulating the development of many organs and tissues. We previously identified Dkk3 to be a molecule predominantly expressed in the mouse embryonic retina. However, which cell expresses Dkk3 in the developing and mature mouse retina remains to be elucidated. To examine the precise expression of the Dkk3 protein, we generated BAC-Dkk3-EGFP transgenic mice that express EGFP integrated into the Dkk3 gene in a BAC plasmid. Expression analysis using the BAC-Dkk3-EGFP transgenic mice revealed that Dkk3 is expressed in retinal progenitor cells (RPCs) at embryonic stages and in Müller glial cells in the adult retina. Since Müller glial cells may play a potential role in retinal regeneration, BAC-Dkk3-EGFP mice could be useful for retinal regeneration studies.