A Novel Bone Morphogenetic Protein 2 Mutant Mouse, , Displays Impaired Intracellular Handling in Skeletal Muscle
Figure 1
The RKR to AAA mutation in the bipartite NLS does not inhibit secretion of the conventional BMP2 growth factor. (a) Detail of the nBMP2 NLS sequence. Critical amino acids in the bipartite NLS are bold and underlined, the furin recognition site is boxed, and the site of cleavage to release the mature secreted growth factor from the propeptide is marked by an arrow. (b) Culture media from cells transfected with either wild type (wtBmp2/HA) or mutant (RKRmBmp2/HA) BMP2 expression plasmids was collected and analyzed by western blot. The HA-tagged growth factors were secreted equally.
