BioMed Research International / 2013 / Article / Tab 2

Research Article

Development of a Novel Reference Plasmid for Accurate Quantification of Genetically Modified Kefeng6 Rice DNA in Food and Feed Samples

Table 2

Optimized concentrations for qPCR and dPCR assays.

ComponentqPCRdPCR
Kefeng6 ( L)gos9  ( L)Kefeng6 ( L)gos9  ( L)

TaqMan Universal Master Mix (2 )12.512.555
Primer-F (10  M)110.40.4
Primer-R (10  M)110.40.4
Probe-P (10  M)0.50.50.20.2
Template ( 20 ng/ L)5522
Loading (10 )0011
H2O5511

Total25251010

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