A Pipeline with Multiplex Reverse Transcription Polymerase Chain Reaction and Microarray for Screening of Chromosomal Translocations in Leukemia
Table 1
Chromosomal alterations included in the multiplex RT-PCR analysis.
Chromosome aberration
Genes involveda
Accession number
Positive controls
Cellsb
Patientsc
Fusion fragments
t(8;21)(q22;q22) AML1-ETO
AML1 (21q22)
D43969
KASUMI-1
ETO (8q22)
D14289
t(15;17)(q22;q21) PML-RARA
PML (15q22)
M73778
NB-4
+ (S form)
RARA (17q21)
X06538
t(11;17)(q23;q21) PLZF-RARA
PLZF (11q23)
Z19002
+
RARA (17q21)
X06538
t(5;17)(q35;q22) NPM1-RARA
NPM1 (5q35)
X16934
+ (S form)
RARA (17q21)
X06538
inv(16)(p13q22) CBFB-MYH11
CBFB (16q22)
L20298
ME-1
+ (type D)
MYH11 (16p13)
D10667
t(12;21)(p13;q22) TEL-AML1
TEL (12p13)
U11732
REH
AML1 (21q22)
D43969
t(1;19)(q23;p13) E2A-PBX1
E2A (19p13)
M31222
+
PBX1 (1q23)
M86546
t(9;22)(q34;q11) BCR-ABL
BCR (22q11)
X02596
K-562 (p210)
+ (p190)
+ (p230)
ABL (9q34)
X16416
del(1)(p32;p32) SIL-TAL1
SIL (1p34)
M74558
+ (type I)
TAL1 (1p34)
S53245
t(4;11)(q21;q23) MLL-AF4
MLL (11q23)
L04284
+
AF4 (4q21)
L13773
t(9;11)(p22;q23) MLL-AF9
MLL (11q23)
L04284
THP-1
AF9 (9p22)
L13744
t(11;19)(q23;p13.3) MLL-ENL
MLL (11q23)
L04284
+
ENL (19p13.3)
D14539
t(11;19)(q23;p13.1) MLL-ELL
MLL (11q23)
L04284
+
ELL (19p13.1)
U16282
t(6;11)(q27;q23) MLL-AF6
MLL (11q23)
L04284
+
AF6 (6q27)
U02478
t(10;11)(p12;q23) MLL-AF10
MLL (11q23)
L04284
+
AF10 (10p12)
U13948
Chromosomes on which genes are located are in brackets. Cells were kindly provided by Ruijin Hospital (Shanghai, China). Patients RNAs were kindly provided by Shanghai Children’s Medical Center (Shanghai, China).