Research Article

A Pipeline with Multiplex Reverse Transcription Polymerase Chain Reaction and Microarray for Screening of Chromosomal Translocations in Leukemia

Table 1

Chromosomal alterations included in the multiplex RT-PCR analysis.

Chromosome aberrationGenes involvedaAccession numberPositive controls
CellsbPatientscFusion fragments

t(8;21)(q22;q22)
AML1-ETO
AML1 (21q22)D43969KASUMI-1
ETO (8q22)D14289
t(15;17)(q22;q21)
PML-RARA
PML (15q22) M73778NB-4+ (S form)
RARA (17q21)X06538
t(11;17)(q23;q21)
PLZF-RARA
PLZF (11q23)Z19002+
RARA (17q21)X06538
t(5;17)(q35;q22)
NPM1-RARA
NPM1 (5q35) X16934+ (S form)
RARA (17q21)X06538
inv(16)(p13q22)
CBFB-MYH11
CBFB (16q22) L20298ME-1+ (type D)
MYH11 (16p13)D10667
t(12;21)(p13;q22)
TEL-AML1
TEL (12p13)U11732REH
AML1 (21q22)D43969
t(1;19)(q23;p13)
E2A-PBX1
E2A (19p13)M31222+
PBX1 (1q23)M86546
t(9;22)(q34;q11)
BCR-ABL
BCR (22q11)X02596K-562 (p210)+ (p190)+ (p230)
ABL (9q34)X16416
del(1)(p32;p32)
SIL-TAL1
SIL (1p34)M74558+ (type I)
TAL1 (1p34)S53245
t(4;11)(q21;q23)
MLL-AF4
MLL (11q23)L04284+
AF4 (4q21)L13773
t(9;11)(p22;q23)
MLL-AF9
MLL (11q23)L04284THP-1
AF9 (9p22)L13744
t(11;19)(q23;p13.3)
MLL-ENL
MLL (11q23)L04284+
ENL (19p13.3)D14539
t(11;19)(q23;p13.1)
MLL-ELL
MLL (11q23)L04284+
ELL (19p13.1)U16282
t(6;11)(q27;q23)
MLL-AF6
MLL (11q23)L04284+
AF6 (6q27)U02478
t(10;11)(p12;q23)
MLL-AF10
MLL (11q23)L04284+
AF10 (10p12)U13948

Chromosomes on which genes are located are in brackets.
Cells were kindly provided by Ruijin Hospital (Shanghai, China).
Patients RNAs were kindly provided by Shanghai Children’s Medical Center (Shanghai, China).