A Pipeline with Multiplex Reverse Transcription Polymerase Chain Reaction and Microarray for Screening of Chromosomal Translocations in Leukemia
Table 2
Chimeric primers used in the multiplex RT-PCR.
Primer name
Primer composition (size)a
Sequence ( to )
ETO_R
D14289_SP6_614 (18)
ATTTAGGTGACACTATAGA GAACTCTTTCTCCTATCT
RARA_R
X06538_SP6_696 (16)
ATTTAGGTGACACTATAGA CGGTCGTTTCTCACAG
MYH11-A_R
D10667_SP6_2271 (18)
ATTTAGGTGACACTATAGA TTGCGTAGCTGCTTGATG
MYH11-DE_R
D10667_SP6_1374 (15)
ATTTAGGTGACACTATAGA GCAGGCTGTTCCGCT
AML1_R
D43969_SP6_731 (17)
ATTTAGGTGACACTATAGA CACGGAGCAGAGGAAGT
PBX1_R
M86546_SP6_657 (19)
ATTTAGGTGACACTATAGA TCGCAGGAGATTCATCACG
TAL1_R
S53245_SP6_257 (15)
ATTTAGGTGACACTATAGA CGTCCCTCTAGCTGG
ABL_R
X16416_SP6_576 (17)
ATTTAGGTGACACTATAGA AGCTGCCATTGATCCCG
AF9_R
L13744_SP6_1910 (20)
ATTTAGGTGACACTATAGA TTCTTGATGCATCCAGTTGT
ENL_R
D14539_SP6_301 (20)
ATTTAGGTGACACTATAGA GACCACCTTCTCCACGAAGT
ELL_R
U16282_SP6_461 (17)
ATTTAGGTGACACTATAGA GTAGCGGCCTCCAGCCT
AF6_R
U02478_SP6_360 (18)
ATTTAGGTGACACTATAGA AATCTGCCTTCCCGATCA
AF10-A_R
U13948_SP6_2384 (19)
ATTTAGGTGACACTATAGA CACTGCCTCTCCAAAAGCT
AF10-B_R
U13948_SP6_1146 (18)
ATTTAGGTGACACTATAGA TGACCTGAGCTGTGAGCT
AF4_R
L13773_SP6_1674 (18)
ATTTAGGTGACACTATAGA TCGAGCATGGATGACGTT
GUS_R
M15182_SP6_2057 (18)
ATTTAGGTGACACTATAGA TGCCGTGAACAGTCCAGG
AML1_F
D43969_T7_903 (22)
TAATACGACTCACTATAGGGA CCAGGTTGCAAGATTTAATGAC
PML-L_F
M73778_T7_1438 (19)
TAATACGACTCACTATAGGGA CAGTGTACGCCTTCTCCATCA
PML-S_F
M73778_T7_927 (25)
TAATACGACTCACTATAGGGA GTGCGCCAGGTGGTAGCTC
PLZF_F
Z19002_T7_1092(21)
TAATACGACTCACTATAGGGA CCACAAGGCTGACGCTGTATT
NPM1_F
X16934_T7_160 (25)
TAATACGACTCACTATAGGGA ACGAAGGCAGTCCAATTAAAGTAAC
CBFB_F
L20298_T7_267 (22)
TAATACGACTCACTATAGGGA TTTGAAGGCTCCCATGATTCTG
TEL_F
U11732_T7_871 (23)
TAATACGACTCACTATAGGGA CACTCCGTGGATTTCAAACAGTC
E2A_F
M31222_T7_1243 (22)
TAATACGACTCACTATAGGGA AAGATAGAAGACCACCTGGACG
SIL_F
M74558_T7_24 (19)
TAATACGACTCACTATAGGGA CGACCCCAACGTCCCAGAG
BCR-190_F
X02596_T7_1590 (20)
TAATACGACTCACTATAGGGA CGCTCTCCCTCGCAGAACT
BCR-210_F
X02596_T7_2952 (24)
TAATACGACTCACTATAGGGA GAGTCACTGCTGCTGCTTATGTC
BCR-230_F
X02596_T7_3682 (19)
TAATACGACTCACTATAGGGA CCAAGGTGCCCTACATCGT
MLL_F
L04284_T7_3916 (20)
TAATACGACTCACTATAGGGA CCGCCTCAGCCACCTACTAC
GUS_F
M15182_T7_1786 (20)
TAATACGACTCACTATAGGGA GGAATTTTGCCGATTTCATG
T7
TAATACGACTCACTATAGGGA
SP6
ATTTAGGTGACACTATAGA
SP6-biotin
biotin-ATTTAGGTGACACTATAGA
_R: reverse primer, _F: forward primer. Primer composition was given with gene accession number plus universal gene (T7 or SP6), the starting position on the gene, and total length in brackets.
