Research Article
A Pipeline with Multiplex Reverse Transcription Polymerase Chain Reaction and Microarray for Screening of Chromosomal Translocations in Leukemia
Table 5
Summary of translocations detected by multiplex RT-PCR-microarray method and clinic diagnostic analysis.
| Translocations | Multiplex RT-PCR-microarrays | Clinic diagnosisa | AML | ALL | CML | AML | ALL | CML |
| t(8;21) AML1-ETO | 8 | | | 8 | | | t(15;17) PML-RARA | 10 | | | 10 | | | inv(16) CBFB-MYH11 | 5 | | | 5 | | | t(9;11) MLL-AF9 | 1 | | | 1 | | | t(11;19) MLL-ENL | 1 | | | 1 | | | t(11;19) MLL-ELL | 1 | | | 1 | | | t(6;11) MLL-AF6 | 1 | | | 1 | | | t(10;11) MLL-AF10 | 1 | | | 1 | | | t(4;11) MLL-AF4 | | 4 | | | 4 | | t(12;21) TEL-AML1 | | 18 | | | 18 | | t(1;19) E2A-PBX1 | | 3 | | | 3 | | t(9;22) BCR-ABL p190 | | 2 | | | 2 | | t(9;22) BCR-ABL p210 | | | 8 | | | 8 | Other translocations | | | | 4 | 3 | | Total number of patients with translocations | 28 | 27 | 8 | 32 | 30 | 8 | Total number of patients | 74 | 115 | 11 | 74 | 115 | 11 |
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Clinic diagnosis was carried out by either cytogenetic, FISH, or RT-PCR analysis.
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