Research Article

A Pipeline with Multiplex Reverse Transcription Polymerase Chain Reaction and Microarray for Screening of Chromosomal Translocations in Leukemia

Table 5

Summary of translocations detected by multiplex RT-PCR-microarray method and clinic diagnostic analysis.

TranslocationsMultiplex RT-PCR-microarraysClinic diagnosisa
AMLALLCMLAMLALLCML

t(8;21) AML1-ETO 88
t(15;17) PML-RARA 1010
inv(16) CBFB-MYH11 55
t(9;11) MLL-AF9 11
t(11;19) MLL-ENL 11
t(11;19) MLL-ELL 11
t(6;11) MLL-AF6 11
t(10;11) MLL-AF10 11
t(4;11) MLL-AF4 44
t(12;21) TEL-AML1 1818
t(1;19) E2A-PBX1 33
t(9;22) BCR-ABL p19022
t(9;22) BCR-ABL p21088
Other translocations43
Total number of patients with translocations2827832308
Total number of patients74115117411511

Clinic diagnosis was carried out by either cytogenetic, FISH, or RT-PCR analysis.