Nonviral Gene Targeting at rDNA Locus of Human Mesenchymal Stem Cells

Figure 1

Effects of VEGF, bFGF, VC, and ITS-X on MSC proliferation. The effects of culture conditions with growth factors alone (a) and (b) in combination were examined on viable cell yield as assayed by MTT. MSC growth curves were generated at plating densities of (c) 1 × 10⁴ cells/cm² and (d) 1 × 10³ cells/cm². (e) Doubling time was calculated (𝑛=3) at these two plating densities. ^#𝑃<0.05. (f) CFU-Fs were stained with crystal violet and captured using a camera (Sony). (g) CFU-Fs efficiency (𝑛=3). (h) The average diameter of CFU-Fs from each set of culture conditions. b, bFGF; V, VEGF; Vc, vitamin C; ITS-X, insulin-transferrin-selenium-X; CM, commercial medium from Stem Cell Technologies; control, DMEM with 10% FBS. *𝑃<0.05.

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