Engineered Hypopharynx from Coculture of Epithelial Cells and Fibroblasts Using Poly(ester urethane) as Substratum
Figure 7
Immunofluorescence of fibroblasts seeded in the bulk (cross-section) of PEU (a) and PEU-SF (b) for 7 d (1) and 14 d (2), respectively. Cell cytoplasm displayed green from vimentin antibody and nucleus displayed blue from DAPI staining under CLSM observation. The seeding density is 5 × 105/mL and 50 μL cell suspension was used for each well. Scale bar 100 μm.
