Engineered Hypopharynx from Coculture of Epithelial Cells and Fibroblasts Using Poly(ester urethane) as Substratum
Figure 8
Immunofluorescence of cocultured epithelial cells (green) and fibroblasts (red). (a1) and (a3) both are surfaces (bottom and top), and (a2) is cross-section. Epithelial cells were seeded on scaffold bottom surface at the density of 5 × 105/mL after fibroblasts were seeded through scaffold’s top face to the bulk at the density of 5 × 105/mL for 7days. 50 μL cell suspension was used for each cell type. The coculture was conducted for another 14 d. Cell cytoplasm of epithelial cell and fibroblast was stained green and red from CK-14 and Vimentin antibody, respectively. The double staining (green first and then red) was performed for the cross-section (a2). The nucleus displayed blue from DAPI staining. The observation was conducted under CLSM. Scale bar 100 μm.
