Proteomic Analysis of Trichinella spiralis Muscle Larval Excretory-Secretory Proteins Recognized by Early Infection Sera
Figure 1
2-DE and Western blot analysis of T. spiralis muscle larval excretory-secretory (ES) proteins. (a) 2-DE gel of ES proteins separated in the first dimension in the pH range 3–10 and then in the second dimension on a 12% polyacrylamide gel. The gel was stained with Coomassie blue R-250, molecular weight standard is on the left, and pI values are indicated. Protein spots selected for analysis are numbered. (b) 2-DE Western blot of ES proteins probed by mouse infection sera at 18 days postinfection (DPI), and the immunoreactive protein spots were detected by the enhanced chemiluminescent (ECL) kit. (c) 2-DE Western blot of ES proteins probed by mouse infection sera at 42 DPI. (d) Western blot map probed by sera from mice before infection.
