BioMed Research International / 2013 / Article / Fig 1

Research Article

Generation of Leukemia Inhibitory Factor-Dependent Induced Pluripotent Stem Cells from the Massachusetts General Hospital Miniature Pig

Figure 1

Generation of iPSCs from porcine ear fibroblast cells. Phase contrast images of PEFs (a) and being reprogrammed cells after 4 days of lentiviral transduction (b). The reprogrammed cells formed colony-like structures after first passage, which were positive for alkaline phosphatase (c). Two lines, A10 at passage 10 (d) and A15 at passage 8 (g), were maintained for the further experiments and showed AP activity ((e) and (h)). Both lines were cultured at a feeder-free condition and then replated onto iMEF feeder. A10 exhibited a flat morphology (f) while A15 formed a dome-shaped colonies with a sharp border (i).
140639.fig.001a
(a)
140639.fig.001b
(b)
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(c)
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(d)
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(e)
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(f)
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(g)
140639.fig.001h
(h)
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(i)

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