Generation of Leukemia Inhibitory Factor-Dependent Induced Pluripotent Stem Cells from the Massachusetts General Hospital Miniature Pig
Figure 1
Generation of iPSCs from porcine ear fibroblast cells. Phase contrast images of PEFs (a) and being reprogrammed cells after 4 days of lentiviral transduction (b). The reprogrammed cells formed colony-like structures after first passage, which were positive for alkaline phosphatase (c). Two lines, A10 at passage 10 (d) and A15 at passage 8 (g), were maintained for the further experiments and showed AP activity ((e) and (h)). Both lines were cultured at a feeder-free condition and then replated onto iMEF feeder. A10 exhibited a flat morphology (f) while A15 formed a dome-shaped colonies with a sharp border (i).
