Generation of Leukemia Inhibitory Factor-Dependent Induced Pluripotent Stem Cells from the Massachusetts General Hospital Miniature Pig
Generation of iPSCs from porcine ear fibroblast cells. Phase contrast images of PEFs (a) and being reprogrammed cells after 4 days of lentiviral transduction (b). The reprogrammed cells formed colony-like structures after first passage, which were positive for alkaline phosphatase (c). Two lines, A10 at passage 10 (d) and A15 at passage 8 (g), were maintained for the further experiments and showed AP activity ((e) and (h)). Both lines were cultured at a feeder-free condition and then replated onto iMEF feeder. A10 exhibited a flat morphology (f) while A15 formed a dome-shaped colonies with a sharp border (i).
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