Figure 1: (a) Alcian blue staining of cultured micromass and quantification. C3H10T1/2 cells were transfected with BMP4 expressing plasmid, pCAGGS-BMP4. Cultured micromass was stained at day 6. The indicated amount of plasmid was supplemented in a 4 mm cuvette (600 μL) at the electroporation. (b) Quantitative RT-PCR for BMP4, Sox9, Aggrecan, and Col2a1 mRNA at day 3. BMP4 mRNA expression levels and chondrogenic properties were increased in the dose dependent manner of pCAGGS-BMP4. , .