Human Mesenchymal Stem Cells Provide Protection against Radiation-Induced Liver Injury by Antioxidative Process, Vasculature Protection, Hepatocyte Differentiation, and Trophic Effects
In situ hybridization analysis and gene expression of hMSCs implanted in liver. (a) To determine whether implanted hMSCs in vivo expressed liver specific genes hMSCs before injection was compared to hMSC gene expression 15 days after injection. RT-PCR values are representative of at least 5 replicates. Results are expressed as a fold increase in gene expression in hMSCs in liver compared to hMSCs before injection. Values greater than one indicate upregulation of the target genes and values less than one indicate their downregulation. (b) Determination of cell fusion. Black arrow indicates human cells beta-2-microglobulin stained. FISH analysis showing the mice transcription centers (white arrow). (Original magnification .) (d) Determination of growth factors secreted by hMSCs implanted in mice liver. The significance for all analyses was set at (**) and (***) and nonsignificance was noted as ns. Each group consisted of 5 animals ().
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