Figure 1: Msh2 silencing in Nth1 +/+ cells. (a) Real-time data analysis for Msh2 gene expression in Nth1 +/+ clones identified by screening. The data is an average of two real-time PCR reactions (each in triplicate) and analyzed using either Actin or Gapdh as the reference gene. (b) Western blot analysis for MSH2 and Gapdh protein expression using whole cell extracts. E is the empty vector control and M1, M2, M3, M6, and M12 are different clones. (c) Quantification of the Western blot band intensity and the data expressed as a percentage of the pshRNAEmpty cell extract. The second blue column marked (−) is Nth1 +/+ cells.