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BioMed Research International
Volume 2013, Article ID 154549, 10 pages
Review Article

Strategies to Characterize Fungal Lipases for Applications in Medicine and Dairy Industry

1Center for Advanced Studies in Botany, University of Madras, Guindy Campus, Chennai, Tamil Nadu 600025, India
2Electronics and Photonics Research Institute, National Institute of Advanced Industrial Science and Technology, Central 5, 1-1-1 Higashi, Tsukuba, Ibaraki 305-8565, Japan
3Department of Biological Engineering, College of Engineering, Inha University, Incheon 402-751, Republic of Korea
4Department of Mathematics, SBK College, Madurai Kamaraj University, Aruppukottai, Tamil Nadu 626101, India

Received 3 May 2013; Accepted 6 June 2013

Academic Editor: Bidur Prasad Chaulagain

Copyright © 2013 Subash C. B. Gopinath et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


Lipases are water-soluble enzymes that act on insoluble substrates and catalyze the hydrolysis of long-chain triglycerides. Lipases play a vital role in the food, detergent, chemical, and pharmaceutical industries. In the past, fungal lipases gained significant attention in the industries due to their substrate specificity and stability under varied chemical and physical conditions. Fungal enzymes are extracellular in nature, and they can be extracted easily, which significantly reduces the cost and makes this source preferable over bacteria. Soil contaminated with spillage from the products of oil and dairy harbors fungal species, which have the potential to secrete lipases to degrade fats and oils. Herein, the strategies involved in the characterization of fungal lipases, capable of degrading fatty substances, are narrated with a focus on further applications.