Simultaneous Quantification of Mitochondrial DNA Damage and Copy Number in Circulating Blood: A Sensitive Approach to Systemic Oxidative Stress
Figure 3
Absolute quantification of total mtDNA and baseline mtDNA damage in lymphocytes and prostate cancer cells. C4-2 (), LNCaP (), and lymphocytes () were analyzed by ss-qPCR for total mtDNA content, damaged mtDNA number, and level of baseline damage. The cell number was calculated from the copy numbers of calicin, a single copy nuclear marker. (a) With mtDNA CO2 marker, the original (CO2 original) and preheated (CO2-heated) DNA templates were quantified for damaged copies and total mtDNA copies, respectively. (b) The baseline damage level was obtained by dividing damaged copies over total copies. (c) Comparison of CO2 versus D-loop markers in lymphocyte samples ().
