Multimolecular Salivary Mucin Complex Is Altered in Saliva of Cigarette Smokers: Detection of Disulfide Bridges by Raman Spectroscopy
Figure 3
SDS-PAGE profiles of saliva proteins from smokers and nonsmokers on a 4–20% gradient gel visualized by Stains-All staining (a), Western blot with MUC5B antibody (b), and CBB protein staining (c). Portions from the same samples were treated with (right panels) and without (left panels) reducing agent DTT. Sizes of standard molecules run in the last lane are shown to the right of the gel. Saliva samples collected under unstimulated (resting) and stimulated conditions are shown in lanes R and S, respectively.
