Downregulation of DLC-1 Gene by Promoter Methylation during Primary Colorectal Cancer Progression

Table 1

Primers for MS-HRMA, HRMA, and real-time PCR.

Usage	Primer	Sequence (5′–3′)	Amplicon (bp)
MS-HRMA	MS-HRM-F	TCGTTACGGTTTTAGAAAGAAA	134
	MS-HRM-R	TTCGCTCCCAACCAAAACATAA
HRMA	KRAS-F	CTGAATATAAACTTGTGGTAGTTGGA	59
	KRAS-R	TATCGTCAAGGCACTCTTGC
	BRAF-F	GGTGATTTTGGTCTAGCTACAG	147
	BRAF-R	AGTAACTCAGCAGCATCTCAGG
Real-time PCR	GAPDH-F	GAAGGTGAAGGTCGGAGTCA	226
	GAPDH-R	GAAGATGGTGATGGGATTTC
	DLC-1-F	ACCTGATCACGCAACAGTGAAACA	191
	DLC-1-R	AGACGCCTGCATAGAGCCTCA