Ovarian Stimulation Affects the Population of Mouse Uterine NK Cells at Early Pregnancy

Figure 2

Photograph of double immunohistochemistry staining of splenic and uterine tissue using antiCD3 and antiCD161 antibodies. The spleen of the control (a) and hyperstimulated pregnant mice: R; red pulp, and W; with pulp. The cytoplasm of the immune cells was observed as blue (white arrow) for NK cells (CD161⁺ and CD3⁻), brown (black arrow) for T cells (CD3⁺), and a mixture of blue and brown color (black arrow head) for NKT cells (CD161⁺ and CD3⁺). The uterine horn of control and hyperstimulated groups, respectively, (c and d) at low magnification and in the previous groups (e and f) at high magnification. The lymphocytes and NK cells were prominent around the blood vessel within the muscular layer (*). M; muscular layer.

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