Over, and Underexpression of Endothelin 1 and TGF-Beta Family Ligands and Receptors in Lung Tissue of Broilers with Pulmonary Hypertension

Table 1

Primers used for RT-qPCR analysis of chicken mRNAs.

GENE	GenBank	PRIMERS 5′-3′	Tm °C	Amplicon bp	Probe
EDN1	XM_418943.3	gccagccagagagacaagaa	60	62	29
		tgagcccagagatcttttcc	59
TGF-β1(TGF-β4)	JQ423909	gtccgggctctgtacaaca	59	87	17
		ccaatactcatcgggtccat	59
TGF-β2	NM_001031045	ccaggttctgaaatccaaaga	59	62	9
		ccactttgctgtcaatgtaacg	60
TGF-β3	NM_205454	gggccctggataccaactac	60	62	25
		aagaggacgcacacagcag	60
TβR I	NM_204246	ggaaattgctcgtcgatgtt	60	65	31
		ggtcataatatggcaactggtaatc	59
TβR II	NM_205428	gagttcaagcaccacgacaa	59	61	7
		cctcggggatcttctcct	59
BAMBI	NM_001195401	acagaggactgcacgatgttt	59	61	12
		ctgctcccttgtcctgaagt	59
ACTB	NM_205518	gctctgactgaccgcgtta	60	60	76
		acgagcgcagcaatatcat	59
GAPDH	NM_204305	gtcctctctggcaaagtcca	60	65	49
		accatgtagttcagatcgatgaag	59