BioMed Research International / 2013 / Article / Tab 3 / Review Article
The Impact of Hepatic Steatosis on Hepatic Ischemia-Reperfusion Injury in Experimental Studies: A Systematic Review Table 3 Liver function tests in experimental models of hepatic warm ischemia-reperfusion injury and hepatic steatosis.
Author Animal Steatosis model % steatosis Type of steatosis Duration of ischemia (mins, type) Duration of reperfusion (mins) Outcome measures Results in steatotic livers (lean livers) Luo et al. [42 ] Mouse HFD + CMDD <30 Mixed 15 (total) 180 ALT AST 450 ± 100 (120 ± 10 IU/L)1 480 ± 100 (210 ± 20 IU/L)1 Domenicali et al. [43 ] Rat2 CDD >30 MaS 60 (total) 120 ALT 7500 ± 500 IU/L Yamada et al. [38 ] Rat D-C 30–60 MiS 30 (partial) 240 ALT 4590 ± 811 (4951 ± 788 IU/L)3 Yamada et al. [39 ] Rat D-C 30–60 MiS 30 (partial) 24 hours ALT 476 ± 244 (368 ± 97 IU/L)3 Ellett et al. [29 ] Mouse HFD 30–60 MaS 35 (total) 24 hours ALT 290 ± 120 (130 ± 30 IU/L) Koti et al. [44 ] Rat2 HC 30–60 MaS 45 (partial) 120 ALT AST 5436.3 ± 984.7 IU/L 3166.3 ± 379.6 IU/L Yamagami et al. [30 ] Rat2 CDD 40–60 MaS 45 (total) 40 or 180 ALT AST 518.7 ± 80.3 IU/L 467.7 ± 185 IU/L He et al. [31 ] Mouse HFD 50–60 MaS 45 (total) 24 hours ALT 180 ± 10 (80 ± 5 IU/L)1 Fusai et al. [45 ] Rabbit2 HC 30–60 MaS 60 (partial) 360 ALT 280 ± 10 U/mL Hafez et al. [46 ] Rabbit2 HC 30–60 MaS 60 (partial) 420 ALT AST 178 ± 34 IU/L 406 ± 86 IU/L Caraceni et al. [32 ] Rat CDD 50–60 MaS 60 (total) 120 ALT 4000 ± 300 IU/L (1000 ± 50 IU/L)1 Marsman et al. [40 ] Rat CMDD >60 MaS 40 (total) 24 hours ALT AST Bili 1500 ± 200 (500 ± 100 IU/L)1 ↑ AST (Data not shown)1 14 ± 3 (6 ± 1
mol/L)1 Selzner et al. [33 ] Mouse CDD >60 Mixed 45 or 60 (partial) 24 hours AST 16566 ± 5731 (500 ± 3395 IU/L)1 Caraceni et al. [34 ] Rat CDD >60 MaS 60 30 to 24 hours ALT 8106 ± 1125 (3872 ± 400 IU/L)1 Hakamada et al. [35 ] Rat CDD >60 MaS 30 or 60 (total) 360 ALT Bili PT 1851.4 ± 66.2 (1861.5 ± 212.6 IU/L)4 0.67 ± 0.1 (0.14 ± 0.02)1 21.0 ± 0.9 (18.4 ± 1.3)4 Rolo et al. [47 ] Rat CDD >60 Mixed 90 (partial) 12 hours ALT AST 1820 ± 5 (1450 ± 20 IU/L)1 1790 ± 100 (1190 ± 100 IU/L)1 Takahashi et al. [37 ] Canine HFD 7–99 MaS 60 24 hours ALT 1400 ± 50 (600 ± 50 IU/L)1
ALT: alanine aminotransferase; AST: aspartate aminotransferase; Bili: bilirubin; CDD: choline-deficient diet; CMDD: choline-methionine-deficient diet; D-C: dextrose with cholesterol; HC: high cholesterol; HFD: high-fat diet; MaS: macrovesicular steatosis; MiS: microvesicular steatosis; Mixed: presence of both macrovesicular and microvesicular steatosis; PT: prothrombin time.
P < 0.05 versus lean livers;
no lean group in study;
no significant difference between steatotic and lean livers;
ALT and PT were significantly higher in steatotic livers compared to lean livers after 30 minutes of ischemia but not after 60 minutes of ischemia.