Table 8: Liver function tests in experimental models of isolated perfused model and hepatic steatosis.

AuthorAnimalSteatosis model% steatosisType of steatosisDuration of ischemia (mins)PerfusateDuration of reperfusion (mins)Outcome measuresResults in steatotic livers
(lean livers)

Jamieson et al. [57]PorcineHFD28Mixed90Soltran48 hoursALT/ASTSimilar ALT/AST
von Heesen et al. [54]RatFFD-C40–50MaS24 hoursHTK60ALT
Bile production
400 ± 100 (20 ± 5 IU/L)1  
250 ± 60 (15 ± 5 IU/L)1  
0 (0.16 ± 0.05  L/g/min)1
Baskin-Bey et al. [55]MouseCMDD>40MaS24 hoursUW60ALT ALT1 (levels not specified)
Caraceni et al. [58]RatCDD50–60MaS18 hoursUW120ALT664 ± 77 (140 ± 30 IU/L)1
Caraceni et al. [59]RatCDD50–60MaS18 hoursUW120ALT64.9 ± 10.7 (6.4 ± 1.7 IU/L)1
Puetz et al. [60]RatFFD-C<60MixednsHTK45ALT↑ ALT1 (levels not specified)
Minor et al. [61]RatFFD-C<60Mixed24 hoursHTK45ALT434.3 ± 70.2 (2.5 ± 0.2 IU/L)1
Arnault et al. [56]RatHFD80–100MiS12, 18, or 24 hoursUW180ALT/AST
Bile production
Bile production2

ALT: alanine aminotransferase; AST: aspartate aminotransferase; CDD: choline-deficient diet; CMDD: choline-methionine-deficient diet; HFD: high-fat diet; FFD-C: fat-free diet enriched with carbohydrate; HTK: histidine-tryptophan-ketoglutarate solution; MaS: macrovesicular steatosis; MiS: microvesicular steatosis; Mixed: presence of both macrovesicular and microvesicular steatosis; ns: not stated; Soltran: Marshall's hypertonic citrate; UW: University of Wisconsin solution.
P < 0.05 versus lean livers; only in livers preserved for 24 hours.