Review Article
The Impact of Hepatic Steatosis on Hepatic Ischemia-Reperfusion Injury in Experimental Studies: A Systematic Review
Table 8
Liver function tests in experimental models of isolated perfused model and hepatic steatosis.
| Author | Animal | Steatosis model | % steatosis | Type of steatosis | Duration of ischemia (mins) | Perfusate | Duration of reperfusion (mins) | Outcome measures | Results in steatotic livers (lean livers) |
| Jamieson et al. [57] | Porcine | HFD | 28 | Mixed | 90 | Soltran | 48 hours | ALT/AST | Similar ALT/AST | von Heesen et al. [54] | Rat | FFD-C | 40–50 | MaS | 24 hours | HTK | 60 | ALT AST Bile production | 400 ± 100 (20 ± 5 IU/L)1 250 ± 60 (15 ± 5 IU/L)1 0 (0.16 ± 0.05 L/g/min)1 | Baskin-Bey et al. [55] | Mouse | CMDD | >40 | MaS | 24 hours | UW | 60 | ALT | ALT1 (levels not specified) | Caraceni et al. [58] | Rat | CDD | 50–60 | MaS | 18 hours | UW | 120 | ALT | 664 ± 77 (140 ± 30 IU/L)1 | Caraceni et al. [59] | Rat | CDD | 50–60 | MaS | 18 hours | UW | 120 | ALT | 64.9 ± 10.7 (6.4 ± 1.7 IU/L)1 | Puetz et al. [60] | Rat | FFD-C | <60 | Mixed | ns | HTK | 45 | ALT | ↑ ALT1 (levels not specified) | Minor et al. [61] | Rat | FFD-C | <60 | Mixed | 24 hours | HTK | 45 | ALT | 434.3 ± 70.2 (2.5 ± 0.2 IU/L)1 | Arnault et al. [56] | Rat | HFD | 80–100 | MiS | 12, 18, or 24 hours | UW | 180 | ALT/AST Bile production | ALT/AST2 Bile production2 |
|
|
ALT: alanine aminotransferase; AST: aspartate aminotransferase; CDD: choline-deficient diet; CMDD: choline-methionine-deficient diet; HFD: high-fat diet; FFD-C: fat-free diet enriched with carbohydrate; HTK: histidine-tryptophan-ketoglutarate solution; MaS: macrovesicular steatosis; MiS: microvesicular steatosis; Mixed: presence of both macrovesicular and microvesicular steatosis; ns: not stated; Soltran: Marshall's hypertonic citrate; UW: University of Wisconsin solution. P < 0.05 versus lean livers; only in livers preserved for 24 hours.
|