Figure 8: Alignment of the amino acid sequence of lactonase from B. weihenstephanensis (accession = KC823046) with other homologs using ClustalW. Lactonase was aligned with AttM lactonase of Klebsiella pneumoniae VA360 (accession code = Zp-23552218), AhlK of Klebsiella pneumonia (accession = AAO47340.1), AiiA of Bacillus cereus (accession code = AAY22194), metallo-beta-lactamase protein from Bacillus cereus (accession = ZP_03103014.1), lactonase of Bacillus thuringiensis serovar sotto (accession = ZP_04127391.1), AiiA of Paenibacillus alvei DSM 29 (accession = ZP_10863318.1), and Aiib from Agrobacterium tumefaciens (accession: 2R2D_E). HXHXDH motif and Tyr (194) were boxed, identical residues were shaded with grey, and the proposed metal ligands according to Thomas and coworkers [22] for the dinuclear zinc form of AHL lactonase were underlined.