Gene Silencing of 4-1BB by RNA Interference Inhibits Acute Rejection in Rats with Liver Transplantation
The pathology of liver in recipient rats with liver transplantation (HE, original magnification 400). The recombination vector of lentivirus contains shRNA targeting the 4-1BB gene was constructed. The liver transplantation was performed using the two-cuff technique. BN recipient rats were infected by the recombinant LVs. The pathology of liver in recipient rats was observed by H-E staining 7 days after liver transplantation. Model: liver transplantation model group; LV-NC: negative-control shRNA group; LV-sh4-1BB1: pcDNA-CMV-sh4-1BB1-Lentivector group.