Gene Silencing of 4-1BB by RNA Interference Inhibits Acute Rejection in Rats with Liver Transplantation

Table 1

Sense and antisense oligomers of shRNA targeting the 4-1BB gene of rats.

shRNA symbol	Target sequence	Start position	shRNA strand
shRNA1	GCTGTTACAAC; ATGGTGGTCA	11	sense; 5′-CACCGCTGTTACAACATGGTGGTCATTCAAGAGATGA; CCACCATGTTGTAACAGCTTTTTTG-3′; antisense; 5′-ACCTTGATTCCAACGGTAACCATCATCTCTTGAAGGA; TACCTTCAAGCATTCCGGAGGATTA-3′
shRNA2	GCAGTAAATAC; CCTCCGGTCT	110	sense; 5′-CACCGCAGTAAATACCCTCCGGTCTTTCAAGAGAAG; ACCGGAGGGTATTTACTGCTTTTTTG -3′; antisense; 5′-AACCCCTAGGGCTTAAGCAATCCAGTCTCTTGAACCA; TTTGGCACTTAAGCCATTGACTGAC-3′
shRNA3	GCAGAGTGTGT; CAAGGCTATT	191	sense; 5′-CACCGCAGAGTGTGTCAAGGCTATTTCAAGAGAAT; AGCCTTCGAGCACACTCTGCTTTTTTG -3′; antisense; 5′-AGGTTACCGGGATCCTTGAACAATACTCTTGAACA; ACCCGGTTTACCAAATACCGGAATTGG-3′
shRNANC	ATTGGACCAAG; TGGTTCATAGC		sense; 5′-CCTGACGATTAAGGACTAGGTCAGCTTCAAGAGACCC; CTTAAAGCTTTCGGACGTCGCGAAC-3′; antisense; 5′-AAGTACCAACTACATCTGGGAACTTCTCTTGAAACCC; TGAATGGGTCATCGTAACTACAAC-3′