BioMed Research International / 2013 / Article / Tab 2

Research Article

Gene Silencing of 4-1BB by RNA Interference Inhibits Acute Rejection in Rats with Liver Transplantation

Table 2

The levels of AST, LDH, ALT, and T-BIL in plasma of BN recipients rats 7 days after liver transplantation.

GroupsALT (U/L)AST (U/L)LDH (U/L)T-BIL (mg/L)

Sham (Control)292.43 ± 32.41428.39 ± 34.66859.34 ± 94.3675.39 ± 8.12
Model580.52 ± 51.96#738.11 ± 75.63#1404.65 ± 168.74#83.94 ± 9.53
LV-NC577.21 ± 61.14784.7 ± 47.851553.19 ± 125.8384.38 ± 10.46
LV-sh4-1BB1333.26 ± 39.92*457.7 ± 43.61*942.81 ± 83.17*76.84 ± 8.43

The recombination vector of lentivirus containing shRNA targeting the 4-1BB gene was constructed. The liver transplantation was performed using the two-cuff technique. BN recipient rats were infected by the recombinant LVs. The blood samples were gathered 7 days after liver transplantation. The liver function markers were tested by using the automatic biochemical meter. Model: liver transplantation model group; LV-NC: negative-control shRNA group; LV-sh4-1BB1: pcDNA-CMV-sh4-1BB-Lentivector1 group. Data were expressed as means ± SD, . # compared with the sham (control) group; * compared with the LV-NC group.