Gene Silencing of 4-1BB by RNA Interference Inhibits Acute Rejection in Rats with Liver Transplantation

Table 2

The levels of AST, LDH, ALT, and T-BIL in plasma of BN recipients rats 7 days after liver transplantation.

Groups	ALT (U/L)	AST (U/L)	LDH (U/L)	T-BIL (mg/L)
Sham (Control)	292.43 ± 32.41	428.39 ± 34.66	859.34 ± 94.36	75.39 ± 8.12
Model	580.52 ± 51.96^#	738.11 ± 75.63^#	1404.65 ± 168.74^#	83.94 ± 9.53
LV-NC	577.21 ± 61.14	784.7 ± 47.85	1553.19 ± 125.83	84.38 ± 10.46
LV-sh4-1BB1	333.26 ± 39.92*	457.7 ± 43.61*	942.81 ± 83.17*	76.84 ± 8.43

The recombination vector of lentivirus containing shRNA targeting the 4-1BB gene was constructed. The liver transplantation was performed using the two-cuff technique. BN recipient rats were infected by the recombinant LVs. The blood samples were gathered 7 days after liver transplantation. The liver function markers were tested by using the automatic biochemical meter. Model: liver transplantation model group; LV-NC: negative-control shRNA group; LV-sh4-1BB1: pcDNA-CMV-sh4-1BB-Lentivector1 group. Data were expressed as means ± SD, 𝑛=8. ^#𝑃<0.05 compared with the sham (control) group; *𝑃<0.05 compared with the LV-NC group.