Gene Silencing of 4-1BB by RNA Interference Inhibits Acute Rejection in Rats with Liver Transplantation

Table 3

The levels of IL-2, IL-10, and IFN-γ in plasma of BN recipients rats 7 days after liver transplantation.

Groups	IL-2 (pg/mL)	IL-10 (pg/mL)	IFN-𝛾 (pg/mL)
Sham (Control)	71.32 ± 8.67	16.85 ± 2.03	52.49 ± 6.75
Model	139.15 ± 13.73^#	15.47 ± 1.27	86.2 ± 8.27^#
LV-NC	142.86 ± 16.58	18.35 ± 2.06	80.3 ± 11.64
LV-sh4-1BB1	78.05 ± 8.64*	16.59 ± 1.85	57.86 ± 8.33*

The recombination vector of lentivirus contains shRNA targeting the 4-1BB gene was constructed. The liver transplantation was performed using the two-cuff technique. BN recipient rats were infected by the recombinant LVs. The blood samples were gathered 7 days after liver transplantation. The concentrations of interleukin-2 (IL-2), IL-10, and interferon-𝛾 (IFN-𝛾) in plasma were tested using enzyme-linked immunosorbent assay (ELISA). Model: liver transplantation model group; LV-NC: negative-control shRNA group; LV-sh4-1BB1: pcDNA-CMV-sh4-1BB-Lentivector1 group. Data were expressed as means ± SD, 𝑛=8. ^#𝑃<0.05 compared with the sham (control) group; *𝑃<0.05 compared with the LV-NC group.